Washington University in St. Louis

New Publication by Anil Cashikar in the Hanson Lab

Congratulation to Anil and colleagues on their new publication in eLife!


Cashikar, AG, Shim, S, Roth, R, Maldazys, MR, Heuser, JE and Hanson, PI  (2014)  Structure of cellular ESCRT-III spirals and their relationship to HIV budding.  eLIFE  [E-published May 30]   Cite as eLife 2014;10.7554/eLife.02184

Abstract:  The ESCRT machinery along with the AAA+ ATPase Vps4 drive membrane scission for trafficking into multivesicular bodies in the endocytic pathway and for the topologically related processes of viral budding and cytokinesis, but how they accomplish this remains unclear. Using deep-etch electron microscopy, we find that endogenous ESCRT-III filaments stabilized by depleting cells of Vps4 create uniform membrane-deforming conical spirals which are assemblies of specific ESCRT-III heteropolymers. To explore functional roles for ESCRT-III filaments, we examine HIV-1 Gag-mediated budding of virus-like particles and find that depleting Vps4 traps ESCRT-III filaments around nascent Gag assemblies. Interpolating between the observed structures suggests a new role for Vps4 in separating ESCRT-III from Gag or other cargo to allow centripetal growth of a neck constricting ESCRT-III spiral.

* To access this article, click here.

** Click here to see a detailed image and description from eLife publication. [Image is featured online July 1, 2014 in Washington University Record]  

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