Washington University in St. Louis

New Piston Lab Publication in Nature Methods

Congratulations to Dave Piston and his lab on their new publication in Nature Methods!


Cranfill, PJ, Sell, BR, Baird, MA, Allen, JR, Lavagnino, Z, de Gruiter, HM, Kremers, GJ, Davidson, MW, Ustione, A and Piston, DW.  (2016)  Quantitative assessment of fluorescent proteins.  Nature Methods, doi: 10.1038/nmeth.3891 [E-published on May 30]

Abstract:  The advent of fluorescent proteins (FPs) for genetic labeling of molecules and cells has revolutionized fluorescence microscopy. Genetic manipulations have created a vast array of bright and stable FPs spanning blue to red spectral regions. Common to autofluorescent FPs is their tight β-barrel structure, which provides the rigidity and chemical environment needed for effectual fluorescence. Despite the common structure, each FP has unique properties. Thus, there is no single 'best' FP for every circumstance, and each FP has advantages and disadvantages. To guide decisions about which FP is right for a given application, we have quantitatively characterized the brightness, photostability, pH stability and monomeric properties of more than 40 FPs to enable straightforward and direct comparison between them. We focus on popular and/or top-performing FPs in each spectral region.

To access the complete journal article, click here.

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