Washington University in St. Louis

New Publication by the Cooper Lab

Congratulations to the Cooper Lab on their new publication in the Journal of Biological Chemistry!

Olivia L. Mooren, Joanna Kim, Jinmei Li and John A. Cooper (2015)  Role of N-WASP in Endothelial Monolayer Formation and Integrity.  Journal of Biological Chemistry  [E-published June 12]

Abstract: Endothelial cells (ECs) form a monolayer that serves as a barrier between the blood and underlying tissue. ECs tightly regulate their cell-cell junctions, controlling the passage of soluble materials and immune cells across the monolayer barrier. We studied the role of N-W ASP , a key regulator of Arp2/3 complex and actin assembly, in EC monolayers. We report that N-WASP regulates endothelial monolayer integrity by affecting the organization of cell junctions. Depletion of N-WASP resulted in an increase in transendothelial electrical resistance, a measure of monolayer integrity. N-WASP depletion increased the width of cell-cell junctions and altered the organization of F- actin and VE-cadherin at junctions. N-W ASP was not present at cell-cell junctions in monolayers under resting conditions, but it was recruited following treatment with sphingosine-1-phosphate. Taken together, our results reveal a novel role for N-WASP in remodeling EC junctions, which is critical for monolayer integrity and function.


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