Washington University in St. Louis

Work by Vivian Lee and Carmen Halabi in the Mecham lab helps explain how a genetic error in the lysyl oxidase gene increases risk of aortic rupture.

Congratulations to Vivian Lee, graduate student in Dr. Robert Mecham's lab for having her work published in the Proceedings of the National Academy of Sciences (PNAS).

Click here for July 18 WUSM News Release to read more on this study.

Vivian S. Lee, Carmen M. Halabi, Erin P. Hoffman, Nikkola Carmichael, Ignaty Leshchiner, Christine G. Lian, Andrew J. Bierhals, Dana Vuzman, Brigham Genomic Medicine, Robert P. Mecham, Natasha Y. Frank, and Nathan O. Stitziel (2016) Loss of function mutation in LOX causes thoracic aortic aneurysm and dissection in humans.  PNAS, [Published online before print July 18, doi: 10.1073/pnas.1601442113]

Abstract:  Thoracic aortic aneurysms and dissections (TAAD) represent a substantial cause of morbidity and mortality worldwide. Many individuals presenting with an inherited form of TAAD do not have causal mutations in the set of genes known to underlie disease. Using whole-genome sequencing in two first cousins with TAAD, we identified a missense mutation in the lysyl oxidase (LOX) gene (c.893T > G encoding p.Met298Arg) that cosegregated with disease in the family. Using clustered regularly interspaced short palindromic repeats (CRISPR)/clustered regularly interspaced short palindromic repeats-associated protein-9 nuclease (Cas9) genome engineering tools, we introduced the human mutation into the homologous position in the mouse genome, creating mice that were heterozygous and homozygous for the human allele. Mutant mice that were heterozygous for the human allele displayed disorganized ultrastructural properties of the aortic wall characterized by fragmented elastic lamellae, whereas mice homozygous for the human allele died shortly after parturition from ascending aortic aneurysm and spontaneous hemorrhage. These data suggest that a missense mutation in LOX is associated with aortic disease in humans, likely through insufficient cross-linking of elastin and collagen in the aortic wall. Mutation carriers may be predisposed to vascular diseases because of weakened vessel walls under stress conditions. LOX sequencing for clinical TAAD may identify additional mutation carriers in the future. Additional studies using our mouse model of LOX-associated TAAD have the potential to clarify the mechanism of disease and identify novel therapeutics specific to this genetic cause.

To access the complete journal article, click here.

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